Review



notch pathway activator jagged1  (Millipore)

 
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 90
      Buy from Supplier

    Structured Review

    Millipore notch pathway activator jagged1
    a – e The proliferation, apoptosis, migration, invasion and stemness of SW480 and HCT116 cells were respectively detected through EdU assay ( a ), flow cytometry ( b ), transwell assay ( c , d ) and sphere formation assay ( e ) in sh-NC, sh-circAGFG1#1, sh-circAGFG1#1+LiCl, sh-circAGFG1#1+SC79 or <t>sh-circAGFG1#1+Jagged1</t> group. f TOP/FOP-flash assay was performed to verify the activity of Wnt/β-catenin pathway in cells transfected with sh-NC or sh-circAGFG1#1. FOP-flash reporter was adopted as the control of TOP flash reporter. g The protein expressions of β-catenin, cyclin D1 and c-myc were measured by western blot in sh-NC or sh-circAGFG1#1 group. h The nuclear translocation of β-catenin in sh-NC or sh-circAGFG1#1 group was assessed by IF assay (scale bar = 20 μm). i Luciferase reporter assay was performed to verify the effect of circAFGF1 knockdown on the activity of CTNNB1 promoter. j The cellular distribution of circAGFG1 was verified by subcellular fraction assay. We repeated the experiments three times to ensure the accuracy of the experiments. ** P < 0.01.
    Notch Pathway Activator Jagged1, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/notch pathway activator jagged1/product/Millipore
    Average 90 stars, based on 1 article reviews
    notch pathway activator jagged1 - by Bioz Stars, 2026-02
    90/100 stars

    Images

    1) Product Images from "CircAGFG1 drives metastasis and stemness in colorectal cancer by modulating YY1/CTNNB1"

    Article Title: CircAGFG1 drives metastasis and stemness in colorectal cancer by modulating YY1/CTNNB1

    Journal: Cell Death & Disease

    doi: 10.1038/s41419-020-2707-6

    a – e The proliferation, apoptosis, migration, invasion and stemness of SW480 and HCT116 cells were respectively detected through EdU assay ( a ), flow cytometry ( b ), transwell assay ( c , d ) and sphere formation assay ( e ) in sh-NC, sh-circAGFG1#1, sh-circAGFG1#1+LiCl, sh-circAGFG1#1+SC79 or sh-circAGFG1#1+Jagged1 group. f TOP/FOP-flash assay was performed to verify the activity of Wnt/β-catenin pathway in cells transfected with sh-NC or sh-circAGFG1#1. FOP-flash reporter was adopted as the control of TOP flash reporter. g The protein expressions of β-catenin, cyclin D1 and c-myc were measured by western blot in sh-NC or sh-circAGFG1#1 group. h The nuclear translocation of β-catenin in sh-NC or sh-circAGFG1#1 group was assessed by IF assay (scale bar = 20 μm). i Luciferase reporter assay was performed to verify the effect of circAFGF1 knockdown on the activity of CTNNB1 promoter. j The cellular distribution of circAGFG1 was verified by subcellular fraction assay. We repeated the experiments three times to ensure the accuracy of the experiments. ** P < 0.01.
    Figure Legend Snippet: a – e The proliferation, apoptosis, migration, invasion and stemness of SW480 and HCT116 cells were respectively detected through EdU assay ( a ), flow cytometry ( b ), transwell assay ( c , d ) and sphere formation assay ( e ) in sh-NC, sh-circAGFG1#1, sh-circAGFG1#1+LiCl, sh-circAGFG1#1+SC79 or sh-circAGFG1#1+Jagged1 group. f TOP/FOP-flash assay was performed to verify the activity of Wnt/β-catenin pathway in cells transfected with sh-NC or sh-circAGFG1#1. FOP-flash reporter was adopted as the control of TOP flash reporter. g The protein expressions of β-catenin, cyclin D1 and c-myc were measured by western blot in sh-NC or sh-circAGFG1#1 group. h The nuclear translocation of β-catenin in sh-NC or sh-circAGFG1#1 group was assessed by IF assay (scale bar = 20 μm). i Luciferase reporter assay was performed to verify the effect of circAFGF1 knockdown on the activity of CTNNB1 promoter. j The cellular distribution of circAGFG1 was verified by subcellular fraction assay. We repeated the experiments three times to ensure the accuracy of the experiments. ** P < 0.01.

    Techniques Used: Migration, EdU Assay, Flow Cytometry, Transwell Assay, Tube Formation Assay, Activity Assay, Transfection, Western Blot, Translocation Assay, Luciferase, Reporter Assay



    Similar Products

    notch pathway activator jagged1  (Millipore)
    90
    Millipore notch pathway activator jagged1
    a – e The proliferation, apoptosis, migration, invasion and stemness of SW480 and HCT116 cells were respectively detected through EdU assay ( a ), flow cytometry ( b ), transwell assay ( c , d ) and sphere formation assay ( e ) in sh-NC, sh-circAGFG1#1, sh-circAGFG1#1+LiCl, sh-circAGFG1#1+SC79 or <t>sh-circAGFG1#1+Jagged1</t> group. f TOP/FOP-flash assay was performed to verify the activity of Wnt/β-catenin pathway in cells transfected with sh-NC or sh-circAGFG1#1. FOP-flash reporter was adopted as the control of TOP flash reporter. g The protein expressions of β-catenin, cyclin D1 and c-myc were measured by western blot in sh-NC or sh-circAGFG1#1 group. h The nuclear translocation of β-catenin in sh-NC or sh-circAGFG1#1 group was assessed by IF assay (scale bar = 20 μm). i Luciferase reporter assay was performed to verify the effect of circAFGF1 knockdown on the activity of CTNNB1 promoter. j The cellular distribution of circAGFG1 was verified by subcellular fraction assay. We repeated the experiments three times to ensure the accuracy of the experiments. ** P < 0.01.
    Notch Pathway Activator Jagged1, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/notch pathway activator jagged1/product/Millipore
    Average 90 stars, based on 1 article reviews
    notch pathway activator jagged1 - by Bioz Stars, 2026-02
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    a – e The proliferation, apoptosis, migration, invasion and stemness of SW480 and HCT116 cells were respectively detected through EdU assay ( a ), flow cytometry ( b ), transwell assay ( c , d ) and sphere formation assay ( e ) in sh-NC, sh-circAGFG1#1, sh-circAGFG1#1+LiCl, sh-circAGFG1#1+SC79 or sh-circAGFG1#1+Jagged1 group. f TOP/FOP-flash assay was performed to verify the activity of Wnt/β-catenin pathway in cells transfected with sh-NC or sh-circAGFG1#1. FOP-flash reporter was adopted as the control of TOP flash reporter. g The protein expressions of β-catenin, cyclin D1 and c-myc were measured by western blot in sh-NC or sh-circAGFG1#1 group. h The nuclear translocation of β-catenin in sh-NC or sh-circAGFG1#1 group was assessed by IF assay (scale bar = 20 μm). i Luciferase reporter assay was performed to verify the effect of circAFGF1 knockdown on the activity of CTNNB1 promoter. j The cellular distribution of circAGFG1 was verified by subcellular fraction assay. We repeated the experiments three times to ensure the accuracy of the experiments. ** P < 0.01.

    Journal: Cell Death & Disease

    Article Title: CircAGFG1 drives metastasis and stemness in colorectal cancer by modulating YY1/CTNNB1

    doi: 10.1038/s41419-020-2707-6

    Figure Lengend Snippet: a – e The proliferation, apoptosis, migration, invasion and stemness of SW480 and HCT116 cells were respectively detected through EdU assay ( a ), flow cytometry ( b ), transwell assay ( c , d ) and sphere formation assay ( e ) in sh-NC, sh-circAGFG1#1, sh-circAGFG1#1+LiCl, sh-circAGFG1#1+SC79 or sh-circAGFG1#1+Jagged1 group. f TOP/FOP-flash assay was performed to verify the activity of Wnt/β-catenin pathway in cells transfected with sh-NC or sh-circAGFG1#1. FOP-flash reporter was adopted as the control of TOP flash reporter. g The protein expressions of β-catenin, cyclin D1 and c-myc were measured by western blot in sh-NC or sh-circAGFG1#1 group. h The nuclear translocation of β-catenin in sh-NC or sh-circAGFG1#1 group was assessed by IF assay (scale bar = 20 μm). i Luciferase reporter assay was performed to verify the effect of circAFGF1 knockdown on the activity of CTNNB1 promoter. j The cellular distribution of circAGFG1 was verified by subcellular fraction assay. We repeated the experiments three times to ensure the accuracy of the experiments. ** P < 0.01.

    Article Snippet: Wnt/β-catenin pathway activator LiCl, AKT activator SC79 and Notch pathway activator Jagged1 were obtained from Sigma-Aldrich (St. Louis, MO, USA).

    Techniques: Migration, EdU Assay, Flow Cytometry, Transwell Assay, Tube Formation Assay, Activity Assay, Transfection, Western Blot, Translocation Assay, Luciferase, Reporter Assay